A method, system and computer program product for determining the mobility of fluorescent particles
Fluorescence recovery after photobleaching
(FRAP) is a widely used technique to measure the mobility and interactions of fluorescently labeled proteins in living cells. However, several limitations restrict its application: First, conventional FRAP has a rather low ~100 ms temporal resolution, which prohibits measurements of faster processes. Second, current FRAP evaluation schemes cannot include spatial constraints imposed by the cellular environment on protein mobility. Third, FRAP in its present form ignores the sequential nature of the bleaching and image acquisition process. In order to overcome the above-mentioned limitations of conventional FRAP DKFZ researchers developed a novel FRAP based method called 3PEA (Pixel-wise Photobleaching Profile Evolution Analysis).
Further Information: PDF
DKFZ (German Cancer Research Center, Deutsches Krebsforschungszentrum)
Phone: +49-6221-42 2955
Contact
Dr. Ruth Herzog
Media Contact
All latest news from the category: Technology Offerings
Newest articles
Magnetic Memory Unlocked with Energy-Efficient MRAM
Researchers from Osaka University introduced an innovative technology to lower power consumption for modern memory devices. Stepping up the Memory Game: Overcoming the Limitations of Traditional RAM Osaka, Japan –…
Next-Level System Security: Smarter Access Control for Organizations
Cutting-Edge Framework for Enhancing System Security Researchers at the University of Electro-Communications have developed a groundbreaking framework for improving system security by analyzing business process logs. This framework focuses on…
How Microbial Life Shapes Lime Formation in the Deep Ocean
Microorganisms are everywhere and have been influencing the Earth’s environment for over 3.5 billion years. Researchers from Germany, Austria and Taiwan have now deciphered the role they play in the…