Bayreuth study on protein biosynthesis in bacteria: New perspectives for antibiotics research

NusG couples transcription and translation. NusG binds to RNA polymerase via its NTD, and to the ribosome via its CTD. It thus serves as a flexible connection between the two machines. Illustration: Philipp K. Zuber

In all living organisms, gene expression is a two-stage process: First, the genetic information stored in the DNA is used as template to synthesize ribonucleic acids, so-called messenger RNAs (mRNAs). By this, genetic information is converted into a form that can be directly used by the cell.

RNA polymerase is responsible for this process, which is called transcription. During the process of translation the messenger-RNAs, in turn, are the molecular blueprints that are read by the ribosome and used for the production of corresponding proteins.

In humans and animals, these two stages of gene expression are separated both spatially and biochemically. In a bacterial cell, in contrast, they are coupled as scientists have known for more than 50 years.

Ten years ago, a research group at the University of Bayreuth led by Prof. Dr. Paul Rösch reported in “Science” first indications that this coupling might actually be mediated by the protein NusG.

Yet only now has the research group led by Dr. Stefan H. Knauer, in cooperation with partners at Columbia University, New York, succeeded in providing the first direct structural evidence.

NusG consists of two flexibly connected domains: an amino-terminal domain (NTD) and a carboxy-terminal domain (CTD). The CTD binds to the ribosome, the NTD to RNA polymerase. Thus, NusG forms a flexible bridge between the central machines of gene expression, similar to the movable coupling between railway wagons.

This connection causes transcription and translation to be synchronised. Key experiments clearly showing this link were performed with high-resolution nuclear magnetic resonance (NMR) spectroscopy and which were carried out at the North Bavarian Centre for High-Resolution NMR Spectroscopy at the University of Bayreuth.

“This opens up highly interesting perspectives for the development of new antibiotic agents. If we can disrupt this molecular bridge, bacterial protein synthesis and thus also reproduction of bacteria will be seriously impaired – most importantly, without affecting the human organism. We are targeting this approach in a current research project, and we have already achieved first promising results,” says Dr. Stefan Knauer.

“We were able to demonstrate the central role of NusG in bacterial protein biosynthesis by combining structural biology, biochemical, and molecular biology methods. We will continue this interdisciplinary approach in the search for efficient antibiotic substances”, adds co-author Philipp Zuber M.Sc., who is doing his PhD thesis at the University of Bayreuth, where he also completed the elite study programme “Macromolecular Science” within the Elite Network of Bavaria.

The study published in “iScience” is the result of a close cooperation between the Bayreuth researchers and the research groups of Prof. Dr. Max Gottesman and Prof. Dr. Joachim Frank at Columbia University in New York. Frank received the Nobel Prize in Chemistry in 2017 for his developments in cryoelectron microscopy, a research technology that was also used in the new study.

Dr. Stefan Knauer
Biochemistry IV – Biopolymers
University of Bayreuth
Phone: +49 (0)921 55-3868
E-mail: stefan.knauer@uni-bayreuth.de

Robert S. Washburn et al.: Escherichia coli NusG links the lead ribosome with the transcription elongation complex. iScience (2020), DOI: https://dx.doi.org/10.1016/j.isci.2020.101352

Media Contact

Christian Wißler Universität Bayreuth

More Information:

http://www.uni-bayreuth.de/

All latest news from the category: Life Sciences and Chemistry

Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Back to home

Comments (0)

Write a comment

Newest articles

High-performance cerium oxide-based thermal switch for efficient heat flow control and sustainable energy systems.

Durable, Efficient, Sustainable: The Rise of Cerium Oxide Thermal Switches

Groundbreaking cerium oxide-based thermal switches achieve remarkable performance, transforming heat flow control with sustainable and efficient technology. Cerium Oxide-Based Thermal Switches Revolutionize Heat Flow Control Thermal switches, which electrically control…

Industrial robots lowering CO2 emissions in manufacturing for sustainable global trade.

How Industrial Robots are Reducing Emissions in Global Manufacturing

A new study explores the intersection of industrial automation and environmental sustainability, focusing on the role of industrial robots in reducing the carbon intensity of manufacturing exports. The research demonstrates…

3D-printed bioceramic grafts for craniomaxillofacial bone regeneration, showcasing precision medicine and patient-specific solutions.

Patients Can Heal Through Precise, Personalized Bioceramic Grafts

A recent review is transforming the landscape of craniomaxillofacial bone regeneration with the introduction of personalized bioceramic grafts. This pioneering research explores the fabrication and clinical potential of synthetic grafts…