Pit-building venom mixers

Researchers from the Max Planck Institute for Chemical Ecology and the University of Giessen report in a new study in Communications Biology, that the adaptation of antlion larvae to their ecological niche has also changed their venom. Antlions inject a complex venom mixture into their prey that differs in composition and effectiveness from the venom of related lacewing larvae and reflects the specific ecology of the species.

In a new study published in Communications Biology, researchers from the Max Planck Institute for Chemical Ecology and the University of Giessen show that the adaptation of antlions to their ecological niche has also changed their venom. They compared the venom system of antlion and closely related green lacewing larvae. Antlions produce a much more complex venom from three different venom glands than lacewing larvae do. All the venom proteins identified come from the insects themselves, not from symbiotic bacteria. Some of the toxins are new and appear to be unique to antlions. Waiting for their victims in pitfall traps in the sand, antlions can use their venom to immobilize larger prey. The venom therefore plays an important ecological role in adapting to their barren habitat.

The larvae of net-winged insects are predators that use venom to catch and digest other arthropods. Well-known members of this order are the families of green lacewings and antlions. Green lacewing larvae are often used as beneficial insects in greenhouses because they feed on aphids and are therefore sometimes called “aphid lions”. Antlions are only found in dry, sandy habitats where they build funnel traps in the sand and wait for insect prey. As there are not many insects in this barren environment, antlions cannot be picky about their prey. The food supply is very limited, so they must overpower and quickly kill even large and defensive prey insects to survive. To do this, they need powerful venom that can effectively paralyze their prey and prevent them from escaping.

Complex venom produced by venom apparatus with three different venom glands

The research team, led by Heiko Vogel from the Max Planck Institute for Chemical Ecology and Andreas Vilcinskas from the University of Giessen, wanted to find out more about antlion venom. In particular, the researchers wanted to know where the venom comes from and whether it is produced by symbiotic bacteria, which organs are responsible for venom production, what the composition of the venom is, and how the venom system and toxicity of the venom differs from that of the related green lacewing larvae.

“We identified a total of 256 venom proteins in the antlion. The complexity of the entire venom apparatus is extraordinary, with three different glands injecting different venoms and digestive enzymes into the prey via the pincers. The antlion’s venom is much more complex and effective than that of the aphid lion, in which we were only able to identify 137 proteins from the venom glands. With the help of genetic analyses, we have also discovered toxins that are apparently unique to antlions”, says first author Maike Fischer.

The research team used a variety of molecular biological, histological and three-dimensional reconstruction methods to study gene expression, protein diversity and the structure of the venom glands. The scientists also used HCR-RNA-FISH, a combination of fluorescence in situ hybridization (FISH) and hybridization chain reaction (HCR), to visualize and measure the distribution and amount of RNA molecules in individual cells. The researchers were able to show that three different tiny venom glands in the antlion are involved in the secretion of venom and produce different venom proteins.

Antlions get by without help from bacteria

Analysis using fluorescence in situ hybridization to visualize bacteria in the tissue also showed that antlions are apparently free of bacterial symbiotic partners. This result surprised the research team. “It is astonishing that antlions have no bacteria in their bodies. On the one hand, this is unusual, as most animals harbor a large number of microorganisms, especially in the gut, some of which are essential for survival. On the other hand, we had expected bacteria in the venom system, as it was previously assumed that certain venom proteins in antlion venom are produced by bacteria”, says Martin Kaltenpoth, who heads the Department of Insect Symbiosis at the Max Planck Institute for Chemical Ecology.

The special venom apparatus is an adaptation to the antlion’s ecological niche

Net-winged insects are of particular interest for biological control, e.g. in greenhouses. The role and evolution of venom composition in different species of net-winged insects has been largely neglected. “Our results show that different habitats and prey spectra have a strong influence on the venom composition and activity of net-winged insects, and that their dynamics could have a major impact on the evolution of predator-prey relationships. Antlions produce a complex venom mixture that enables them to overwhelm even large, defensive insects in their prey-poor habitat. In addition, they have developed evolutionarily unique structures that allow them to inject either venom or digestive enzymes into their prey via their mouthparts in separate systems,” says Heiko Vogel, head of the “Adaptation and Immunity” project group in the Department of Insect Symbiosis.

Andreas Vilcinskas from the University of Giessen, head of the “Bioresources” department at the Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), continues to keep an eye on the effectiveness and complexity of the antlion venom: “We were able to show that antlion venom is highly effective when injected into insects. However, we don’t know which substances are responsible for this toxicity. It would be interesting to find out which components play which roles in this very complex venom and how it differs from other insect venoms. The toxins found only in the antlion are particularly interesting”, he says, looking ahead to further research.

Wissenschaftliche Ansprechpartner:

Dr. Heiko Vogel, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745 Jena, Germany, Tel. +49 3641 57-1512, E-Mail hvogel@ice.mpg.de
Prof. Dr. Andreas Vilcinskas, Institute of Insect Biotechnology, Justus-Liebig-Universität Gießen, Heinrich-Buff-Ring 26-32, 35392 Gießen, Germany, Tel.: +49 641 99-37600, E-Mail Andreas.Vilcinskas@agrar.uni-giessen.de

Originalpublikation:

Fischer, M. L., Schmidtberg, H., Tidswell, O., Weiss, B., Dersch, L., Lüddecke,T., Wielsch, N., Kaltenpoth, M., Vilcinskas, A., Vogel, H. (2024). Divergent venom effectors correlate with ecological niche in neuropteran predators. Communications Biology, 7: 981, doi: 10.1038/s42003-024-06666-9
https://doi.org/10.1038/s42003-024-06666-9

Weitere Informationen:

https://www.ice.mpg.de/219957/host-plant-adaptation Project Group Host Plant Adaptation and Immunity