Tufts Medical Center researchers finds marker in premies' saliva predicts readiness to feed by mouth

Tufts Medical Center researchers have shown that presence of a gene strongly linked to appetite regulation is highly predictive of a premature infant's readiness to feed orally. An analysis of just a drop of an infant's saliva could be the key to preventing many feeding problems and the expensive medical complications that can occur when infants are fed by mouth too early.

In a study published in the May 21 edition of the journal PLoS ONE, Maron and colleagues have identified a biomarker in saliva that predicts a baby is not yet ready to feed 95 percent of the time. The biomarker, a gene for the neuropeptide Y2 receptor, NPY2R, is a known regulator of feeding behavior. In their study, the researchers demonstrated that levels of NPY2R in saliva decline as a newborn matures enough to feed orally.

“There's a really important need for a better understanding and a more accurate assessment of infants' feeding skills, '' said Jill L. Maron, MD, MPH, a researcher at the Mother Infant Research Institute at Tufts Medical Center. “Nearly every baby born early is at risk for feeding associated morbidities, which often lead to prolonged hospitalizations, short and long term health complications, and significant parental anxiety. This is a way of monitoring the most vulnerable babies very non-invasively. We can help guide clinical care without ever hurting them.''

Currently, caregivers use a variety of subjective measurements, such as evaluating a baby's sucking and swallowing skills, to determine when it's safe to feed a baby by mouth. But these methods are imprecise and often lead to feeding a baby too early, which can cause the child to choke, accidentally inhale breast milk or formula into their lungs leading to pneumonia, or other problems. Babies who suffer these early feeding difficulties can also go on to develop long-term feeding problems and are at risk of developmental delays. Research indicates that more than 40 percent of children in feeding disorder clinics were premature babies.

The NPY2R gene has been studied extensively because it helps regulate appetite and plays a role in both obesity and eating disorders. But no one had examined its role in prompting premature babies to eat, because most researchers were not focusing on appetite's role in newborn feeding problems.

“No one had targeted this area of research because you can't ask a baby, are you hungry?” said Maron. But, in fact, premature babies need to grow to three or four times their initial birth weight in the first year of life. This requires a voracious appetite and while researchers aren't sure exactly how it works, it appears that NPY2R suppresses that appetite.

“More research is needed to explain the biological mechanism regulating this gene in infants. However, if we can detect NPY2R in newborns' saliva, there's a 95 percent chance they won't feed for you or they will be poor feeders,'' said Maron.

Maron is developing a simple saliva test for use in neonatal intensive care units to help doctors determine that it's safe to start transitioning premature babies to oral feeding. Feeding is a complex behavior that involves a variety of skills, muscles and nerves. Ultimately, Maron hopes to develop a comprehensive panel that would help determine not only when babies are ready to feed, but identify the factors that are limiting their feeding success. Such an assay could shorten hospital stays, reduce significant morbidities and save millions in healthcare costs.

Maron, Jill, “Neuropeptide Y2 Receptor (NPY2R) Expression in Saliva Predicts Feeding Immaturity in the Premature Neonate,” published online on May 21, can be viewed at http://dx.plos.org/10.1371/journal.pone.0037870.