Clue to prion formation found, offers step toward treating puzzling diseases

Prions—their existence is intriguing and their links to disease are unsettling. These unconventional infectious agents are involved in mad cow disease and other fatal brain illnesses in humans and animals, rattling prior assumptions about the spread of infections.

Dartmouth Medical School biochemists studying the mysteries of these prion particles have discovered a novel step in their formation. Their results, reported in a recent issue of Biochemistry could help provide a new approach for therapy against prion diseases. The team, headed by Dr. Surachai Supattapone, assistant professor of biochemistry and of medicine, includes Ralf Lucassen and Koren Nishina.

The cause of certain neurodegenerative diseases has long stymied scientists. The variant Creutzfeldt-Jakob disease in humans — which is linked to bovine spongiform encephalopathy or mad cow disease — as well as scrapie in sheep and chronic wasting disease in deer and elk are transmissible.

Yet the infectious agent is not a parasite, fungus, bacterium or virus. Instead, it seems to be a prion, which is a protein, but an abnormally shaped one. A normal brain protein called PrPC misfolds into the prion protein called PrPSc. Until prions were discovered, proteins were not considered agents of infection.

Still unknown is how PrPC turns into PrPSc, which is characterized biochemically by being resistant to enyzme digestion. The researchers created a system to study the conversion into PrPSc in a test tube.

“We found that we could inhibit this conversion with compounds that block free sulfhydryl groups,” said Supattapone. “This is the first discovery that formation of PrPSc requires a reactive chemical group. It is a clue that there may be a cofactor containing the free sulfhydryl group, such as an enzyme that helps to catalyze the process of forming PrPSc from PrPC. Moreover, it may be a first step of a logical approach to find a therapeutic strategy against prion disease based on specifically inhibiting a catalytic cofactor.”

Media Contact

Andy Nordhoff EurekAlert!

More Information:

http://www.dartmouth.edu/

All latest news from the category: Life Sciences and Chemistry

Articles and reports from the Life Sciences and chemistry area deal with applied and basic research into modern biology, chemistry and human medicine.

Valuable information can be found on a range of life sciences fields including bacteriology, biochemistry, bionics, bioinformatics, biophysics, biotechnology, genetics, geobotany, human biology, marine biology, microbiology, molecular biology, cellular biology, zoology, bioinorganic chemistry, microchemistry and environmental chemistry.

Back to home

Comments (0)

Write a comment

Newest articles

Future AR/VR controllers could be the palm of your hand

Carnegie Mellon University’s EgoTouch creates simple interfaces for virtual and augmented reality. The new generation of augmented and virtual reality controllers may not just fit in the palm of your…

‘Game changer’ in lithium extraction

Rice researchers develop novel electrochemical reactor. A team of Rice University researchers led by Lisa Biswal and Haotian Wang has developed an innovative electrochemical reactor to extract lithium from natural…

The blue-green sustainable proteins of seaweed

… may soon be on your plate. The protein in sea lettuce, a type of seaweed, is a promising complement to both meat and other current alternative protein sources. Seaweed…