Method of determining the degradation of cellulose materials
The constantly growing global population and the resulting increase in energy consumption, the foreseeable end of the global petroleum reserves and a need for stagnation or rather a decline of the emissions of greenhouse gas led to a closer focus on alternative energy sources. Especially the production of biofuel out of renewable, cellulosic raw material is constantly being optimized at the moment. One major challenge in this context is the generation of new, highly effective cellulolytic enzymes. Our novel assay, called hydrogel reagent signaling system (HyReS), offers significant advantages, as it enables a straightforward method for analyzing cellulolytic enzymes with a spatially-resolved imaging of cellulose hydrolysis in real-time using a simple fluorescent readout.
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