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New peptides for the prevention and treatment of atherosclerosis
Further Information: PDF PROvendis GmbHPhone: +49 (0)208/94105 0ContactDipl.-Ing. Alfred…
The innovation discloses a novel genetic technique in the area of applied immunology and oncology. With that approach we were able to harness human T-cells…
The invention at hand provides a novel protein for the chemoenzymatic and enantiomerically pure production of L-<i>threo</i>-hydroxyaspartate from L-aspartate.<p>
The naturally occurring protein AsnO (asparagine oxygenase) is part of the CDA biosynthesis gene cluster in Streptomyces coelicor. AsnO is an Fe<sup>2+</sup> and <font face=”symbol”>a</font>-ketoglutarate-dependent hydroxylase. This hydroxylase acts as a building block for non-ribosomally produced CDA (?Calcium-dependent antibiotic?) in vivo. During the catalytic cycle, this class of enzymes couples the oxidative decomposition of <font face=”symbol”>a</font>-ketoglutarate to succinate and CO<sub>2</sub> with the hydroxylation of the substrate (L-asparagine).<p>
In the wild type of AsnO, the side chain of the amino acid residue Asp-241 thereby binds to the NH<sub>2</sub> group of the carboxamide group of L-Asn.
Surprisingly, it was found that directed mutagenesis of Asp-241 to Asn-241 (D241N) results in a binding site for the carboxyl group of an aspartate side chain. By means of this directed mutagenesis, the substrate specificity changes from asparagine to aspartate, and the mutated protein converts aspartate chemoenzymatically to L-threo-hydroxyaspartate in the presence of Fe2+ and a-ketoglutarate.<p>
Thereby, AsnO D241N represents the mutant of the wild type AsnO according to the present invention.
The invention is a procedure to reduce the bit fault rate in UMTS networks and thus to allow to process more data simultaneously, to reduce the required…
In industrial applications of MIG welding of aluminium usually wires with a diameter of 1.0 mm to 1.6 mm are used. Filler metals with smaller wire diameters…
The generation of protons by irradiating thin foils by ultra short laser pulses (laser-driven proton beams) has recently
gained enormous attention. These…