Protein P4 (SLC10A4) as a marker protein for cholinergic neurons of the CNS
The protein SLC10A4, also known as P4, occurs in numerous cholinergic neurons. Up to now, however, a method that allows for the detection of SLC10A in cholinergic neurons does not exist.<p>
The invention at hand discloses a procedure for the detection of cholinergic neurons in the CNS by means of the detection of SLC10A4. For this purpose, oligopeptides of the C terminus of SLC10A4 are coupled to KLH, and polyclonal antibodies against these antigens are subsequently formed. According to the present invention, polyclonal antibodies against SLC10A4 thus obtained are used for the detection of SLC10A4 in cholinergic neurons, for example by means of the sandwich method. The visualization of SLC10A4 can be carried out, for instance, via immunofluorescence or peroxidase staining.
Further Information: PDF
TransMIT Gesellschaft für Technologietransfer mbH
Phone: +49 (0)641/943 64-12
Contact
Dr. Peter Stumpf
Media Contact
All latest news from the category: Technology Offerings
Newest articles
You are What You Eat—Stanford Study Links Fiber to Anti-Cancer Gene Modulation
The Fiber Gap: A Growing Concern in American Diets Fiber is well known to be an important part of a healthy diet, yet less than 10% of Americans eat the minimum recommended…
Trust Your Gut—RNA-Protein Discovery for Better Immunity
HIRI researchers uncover control mechanisms of polysaccharide utilization in Bacteroides thetaiotaomicron. Researchers at the Helmholtz Institute for RNA-based Infection Research (HIRI) and the Julius-Maximilians-Universität (JMU) in Würzburg have identified a…
ASXL1 Mutation: The Hidden Trigger Behind Blood Cancers and Inflammation
Scientists show how a mutated gene harms red and white blood cells. LA JOLLA, CA—Scientists at La Jolla Institute for Immunology (LJI) have discovered how a mutated gene kicks off…