T7 promoter
Cell-free expression systems are widely used for RNA and protein production. The majority of systems available are based on the T7 RNA polymerase together with the wild type T7 promoter.
By an in vitro evolution approach we could create a completely new variant of the T7 promoter, which shows increased transcriptional activity (10-fold) and 2-fold higher protein expression levels under standard conditions. In addition to the increased yields faster reaction conditions were also observed for the Turbo T7 promoter.
Further Information: PDF
rubitec – Gesellschaft für Innovation und Technologie der Ruhr-Universität Bochum mbH
Phone: +49 (0)234/32-11969
Contact
Dietmar Tappe
Media Contact
All latest news from the category: Technology Offerings
Newest articles
First-of-its-kind study uses remote sensing to monitor plastic debris in rivers and lakes
Remote sensing creates a cost-effective solution to monitoring plastic pollution. A first-of-its-kind study from researchers at the University of Minnesota Twin Cities shows how remote sensing can help monitor and…
Laser-based artificial neuron mimics nerve cell functions at lightning speed
With a processing speed a billion times faster than nature, chip-based laser neuron could help advance AI tasks such as pattern recognition and sequence prediction. Researchers have developed a laser-based…
Optimising the processing of plastic waste
Just one look in the yellow bin reveals a colourful jumble of different types of plastic. However, the purer and more uniform plastic waste is, the easier it is to…